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| Aton, Emilie; Renault, Tristan; Gagnaire, Beatrice; Thomas Guyon, Hélène; Cognard, C; Imbert, N. |
| Bivalve haemocytes are essential in defence mechanisms including phagocytosis. They also produce molecules including hydrolytic enzymes and antimicrobial peptides that contribute to pathogen destruction. Although haemocyte activities have been extensively studied, relatively little is known about the intracellular signalling pathways that are evoked during haemocyte activation and especially the role of calcium. Flow cytometry has been used for the first time to define the effect of cell incubation in haemolymph and artificial sea water (ASW) on Pacific oyster, Crassostrea gigas, haemocytes. Cell viability, enzymatic activities (esterases and amino peptidases), phagocytosis and granulocyte percentage were analysed. Viability and some activities were... |
| Tipo: Text |
Palavras-chave: Cellular activities; Fluo 3/AM; Calcium; Flow cytometry; Haemocytes; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-935.pdf |
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| Aton, Emilie. |
| Les cultures de mollusques bivalves sont continuellement exposées à des variations de facteurs d'origine naturelle (variation de la salinité et de température) ou anthropique (métaux lourds, HAP, phytosanitaires) susceptibles d'altérer les systèmes de défense des mollusques bivalves. Ces altérations expliqueraient, dans certains cas, leur sensibilité vis à vis de certains pathoègnes marins et pourraient, en partie, être à l'origine des épisodes de mortalité massive observés périodiquement chez des populations larvaires d'huîtres et de moules. C'est pourquoi de nombreuses études concernant l'immunotoxicité des polluants sont actuellement en cours. Le calcium est un second messager ubiquitaire connu pour assurer la transduction de très nombreux signaux... |
| Tipo: Text |
Palavras-chave: Huître creuse; Crassostrea gigas; Hémocytes; Calcium; Transfert calcique; Impact polluant. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00032/14336/11615.pdf |
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